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Cleanup of TRIzol® Reagent-Purified Total RNA Using the PureYield™ RNA Midiprep System
Michelle Mandrekar, M.S., Paula Brisco, M.S.,
Katharine Hoffmann, B.S., and Rex Bitner, Ph.D.
Promega Corporation
The PureYield™ RNA Midiprep System uses a novel Clearing Agent and method
to purify RNA that is essentially free of genomic DNA contamination and
ready for use in downstream applications. The PureYield™ RNA Midiprep
System can also be used to clean up phenol:chloroform-extracted RNA
samples, such as those purified using TRIzol® reagent. In
addition to removal of residual RNases in extracted RNA, the PureYield™
RNA Midiprep System also removes DNA contaminants from the samples.
Removal of DNA contamination is critical for sensitive applications,
such as quantitative RT-PCR and microarray analysis.
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