|
Download
article
231kb
pdf? |
|
Optimizing Kinase Assays for Ultrahigh-Throughput Profiling
Using the Kinase-Glo® Plus Assay
Tracy Worzella, M.S., and Brad Larson, B.S.
Promega Corporation
Here we demonstrate the concept of kinase profiling using a panel of four
kinases and ten kinase inhibitors. We used the Kinase-Glo®
Plus Luminescent Kinase Assay and the Deerac Fluidics™ Equator™ HTS
non-contact liquid handling system to design and perform an
ultrahigh-throughput profiling screen. We present IC50 values
for each test compound and show how such profiling can streamline the
initial drug discovery process.
|
