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TNT® SP6 High-Yield Protein Expression System: More
Protein from a Coupled Transcription/Translation System
Robin Hurst, M.S., Don Creswell, B.S., Michael
Slater, Ph.D., and Elaine Schenborn, Ph.D.
Promega Corporation
The TNT® SP6 High-Yield Protein Expression System is designed
to produce high levels of protein in a single-tube, cell-free format.
This system is based on a supplemented wheat germ extract, containing
all the components required for coupled SP6 RNA polymerase-dependent
transcription and translation. This article compares protein yields from
the TNT® SP6 High-Yield System to the conventional wheat germ
coupled system. We also demonstrate that the TNT® SP6
High-Yield System performs well in combination with Flexi®
Vectors designed for protein expression in wheat germ extracts.
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