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PureYield™ RNA Midiprep System: Isolating Pure Total RNA
without DNase
Paula Brisco, M.S., Michelle Mandrekar, M.S., Rex
Bitner, Ph.D., Katharine Hoffmann, B.S., and Nadine Nassif, M.S.
Promega Corporation
The PureYield™ RNA Midiprep System is designed to isolate high-quality
total RNA with high yields from essentially any type of sample.
Efficient DNA removal is integrated into a streamlined, universal
protocol and is accomplished without DNase treatment or
phenol:chloroform extractions. This novel approach results in pure total
RNA that is essentially free of genomic DNA. The purified total RNA is
ready for use in downstream applications, such as qRT-PCR and microarray
analysis.
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