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The Plexor™ Systems Provide Accurate Quantitation in Multiplex
qPCR and qRT-PCR
Susan Frackman, Ph.D., Benjamin Krenke, M.S.,
Katharine Hoffmann, B.S., Cindy Sprecher, B.S., Kyle Hooper, Ph.D.,
Doug Storts, Ph.D.
Promega Corporation
The power of the polymerase chain reaction (PCR) is greatly enhanced by
techniques that provide accurate, sensitive and reproducible
quantitative data. These real-time or quantitative PCR (qPCR) techniques
determine the amount of input template based on the accumulation of PCR
product during the exponential phase of an amplification reaction. In
this article we introduce the Plexor™ Systems, which use a novel
technique for real-time PCR that that requires only two primers for
sensitive and specific quantitation.
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