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Metal Affinity Tag for Protein Expression and Purification
using the Flexi® Vectors
Becky Godat, M.A., Jim Hartnett, M.S., Jami
English, M.S., Laurie Engel, B.S., Michael Slater, Ph.D., Tonny
Johnson, Ph.D., and Elaine Schenborn, Ph.D.
Promega Corporation
We have developed a new metal affinity tag, HQ tag, for the purification
of recombinant proteins. The HQ tag is incorporated into several Flexi®
Vectors for convenient cloning and expression. Protein-coding regions
can be easily and efficiently transferred into this affinity-tag vector
from other Flexi® Vectors. The HQ Flexi® Vectors
come in four formats to provide flexibility in tag location and
antibiotic resistance. The HQ tag performs similarly to a polyhistidine
tag purified from bacterial cells. However, some HQ-tagged proteins can
be eluted from the affinity columns at lower concentrations of imidazole.
This property of the HQ tag may be useful for downstream applications
such as enzymatic reactions.
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