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Using Bioluminescent Reporter Genes to Optimize shRNA Target
Sites for RNAi of the bcr/abl Gene
Natalie Betz, Ph.D.
Promega Corporation
Not all shRNAs are equally effective at suppressing target gene expression
in mammalian cells. Therefore it is important to quickly and easily
identify the shRNA sequence that is the most effective at inducing RNA
interference for a particular target gene sequence. The psiCHECK™-2
Vector, which contains two luminescent reporter genes, was used to
identify the most effective shRNA for inducing RNAi of the bcr/abl
gene. The seven shRNAs tested were generated using the T7 RiboMAX™
Express RNAi System.
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