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HisLink™ 96 Protein Purification System: Fast Purification of Polyhistidine-Tagged Proteins

Laurie Engel, B.S., Rod Flemming, Ph.D., Tonny Johnson, Ph.D., Cristopher Cowan, Ph.D., Natalie Betz, Ph.D., and Brian Almond, Ph.D.
Promega Corporation

The HisLink™ 96 Protein Purification System allows efficient purification of polyhistidine-tagged proteins from 1ml of culture using a vacuum-based format. The system purifies polyhistidine-tagged proteins directly from bacterial, insect and mammalian cell culture without the need for prior processing of the culture or resin. Removing these prior processing steps results in a robust purification method that is easy to automate and adaptable to rapid screening processes.

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Promega Notes 90 (2005) 15–18: Request this issue.
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