Download
article
168kb

pdf?

Measuring Renilla Luciferase Luminescence in Living Cells

Erika Hawkins¹, M.S., James Unch², Ph.D., Nancy Murphy¹, B.S.,
Jolanta Vidugiriene¹, Ph.D., Mike Scurria², Dieter H. Klaubert², Ph.D., and
Keith V. Wood¹, Ph.D.
¹Promega Corporation and ²Promega Biosciences, Inc.

The ViviRen™ and EnduRen™ Live Cell Substrates have been developed to make measuring Renilla luciferase from live cells simpler and more sensitive than was possible with the substrates coelenterazine and coelenterazine-h. ViviRen™ Substrate generates very bright luminescence, at least 3-fold brighter than coelenterazine, while EnduRen™ Substrate generates very stable luminescence, permitting multiple measurements over incubations of 24 hours or more. Both live cell substrates can be multiplexed with CellTiter-Glo^® Reagent to normalize for viable cell numbers. Alternatively, the Chroma-Luc™ red Luciferase can be used to normalize for viable cell number or transfection efficiency.
 

Promega Notes 90 (2005) 10–14: Request this issue.
Index for Promega Notes 

Printed publications disclaimer.