|
Download
article
319kb
pdf? |
|
Pronto!™ Plus Systems: New Integrated Reagent Systems for
Optimal Microarray Performance
Camilo Canel, Ph.D.1, Thomas A. Bunch, M.S.1, and Nadine Nassif, M.S.2
1Corning Life Sciences, 2Promega Corporation
Promega Corporation and Corning Incorporated have developed two new
systems that provide fully integrated sets of reagents for use in
microarray analysis. The Pronto!™ Plus Direct System provides reagents
for generating fluorescent cDNA by direct incorporation of Cy®-labeled dCTP. The Pronto!™
Plus Indirect System allows users to generate
fluorescent cDNA via synthesis of aminoallyl-modified cDNA, followed by
post-synthesis conjugation of CyDye™ NHS ester. Both systems provide
reagents and protocols specifically optimized for microarray
applications and include components for cDNA labeling and cDNA clean-up
as well as solutions for array conditioning and hybridization. Users can
also choose versions of both systems that include the SV Total RNA
Isolation System, which provides a fast and simple procedure for
purifying intact total RNA from a variety of sources. These integrated
sets of reagents enable researchers to achieve the highest possible
quality, consistency, and reproducibility throughout their microarray
processes. Here we describe the system components and present typical
data generated using these systems.
|
