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Plexor™ Technology: A New Chemistry for Real-Time PCR
Susan Frackman, Ph.D., Steve Ekenberg, Ph.D., Katharine Hoffmann, B.S., Benjamin Krenke, M.S., Cynthia Sprecher, B.S., and Douglas Storts, Ph.D.
Promega Corporation.
The power of the polymerase chain reaction (PCR) is greatly enhanced by
techniques that accurately, sensitively and reproducibly quantify DNA
samples. These real-time or quantitative PCR (qPCR) techniques rely on
the ability to detect the PCR product at each cycle during the
exponential phase. Here we describe the Plexor™ technology, a novel
technique for real-time PCR that requires only two primers for sensitive
and specific quantification.
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