|
Download
article
248kb
pdf? |
|
GeneClip™ U1 Hairpin Cloning Systems for Expression of Short
Hairpin RNAs in vivo
By Thomas Yeager, Ph.D., Cheryl Bailey, Ph.D.,
Jolanta Vidugiriene, Ph.D., Brian Almond, Ph.D., and Doug Storts,
Ph.D., Promega Corporation
The GeneClip™ U1 Hairpin Cloning Systems enable cloning and expression of
short hairpin RNAs (shRNAs) for RNA interference (RNAi) experiments. The
GeneClip™ Systems contain linearized vectors that facilitate cloning of
complementary oligonucleotides downstream of a U1 promoter. Upon
transfection with pGeneClip™ Vectors containing these cloned sequences,
shRNA is produced in the cell, leading to reduction of target gene
expression through RNAi. In this article we illustrate use of the
GeneClip™ Systems to suppress Renilla luciferase expression in a range
of cell types and to suppress p53 expression in both a transient and
stable manner.
|
