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A New System for Cloning and Expressing Protein-Coding Regions
By Michael Slater, Ph.D., Jim Hartnett, M.S.,
Natalie Betz, Ph.D., Jami English, M.S., Ethan Strauss, Ph.D., Becky
Pferdehirt, B.S., and Elaine Schenborn, Ph.D., Promega Corporation
The Flexi® Vector Systems provide a rapid, efficient and
high-fidelity method to transfer protein-coding regions between vectors
with various expression or peptide tag options. Inserts are efficiently
transferred between Flexi® Vectors following restriction
enzyme digestion, maintaining insert orientation and reading frame.
During the initial cloning step, a lethal gene in the Flexi®
Vector cloning region allows positive selection for ligation of the
insert. Antibiotic resistance genes simplify selection of the desired
clone during subsequent transfer to other Flexi® Vectors.
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