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pGL4 Vectors: A New Generation of Luciferase Reporter Vectors
By Aileen Paguio, M.S., Brian Almond, Ph.D., Frank
Fan, Ph.D., Pete Stecha, B.S., Denise Garvin, M.S., Monika Wood,
M.S., and Keith Wood, Ph.D.,
Promega Corporation
Reporter gene technologies are powerful tools by which to study gene
expression and regulation. Among reporters used in mammalian cells, the
luciferases are preferred because they are highly sensitive, flexible,
easy to quantify and detectable over a broad dynamic range. We have
developed several new reporter designs for firefly and Renilla
luciferases, which allow a range of uses from measurement of
transcriptional activity in cell biology applications to high-throughput
screening in drug discovery. The resulting vectors yield higher
expression with decreased signal-to-background ratios and respond more
rapidly to transcriptional dynamics. The vectors are also designed to
reduce the risk of anomalous effects.
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