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HaloTag™ Interchangeable Labeling Technology for Cell Imaging,
Protein Capture and Immobilization
By Georgyi V. Los, Ph.D.1, Al Darzins, Ph.D.1,
Chad Zimprich, B.S.1, Natasha Karassina, M.S.1, Randall Learish,
Ph.D.1, Mark G. McDougall, Ph.D.2, Lance P. Encell, Ph.D.1, Rachel
Friedman-Ohana, Ph.D.1, Monika Wood, M.S.1, Gediminas Vidugiris,
Ph.D.1, Kris Zimmerman, B.S.1, Paul Otto, M.S.1, Dieter H. Klaubert,
Ph.D.,2 and Keith Wood, Ph.D.1; 1Promega Corporation,
2Promega
Biosciences, Incorporated
The HaloTag™ Interchangeable Labeling Technology is designed to provide
new options for rapid, site-specific labeling of proteins in living
cells and in vitro. The technology is based on the efficient formation
of a covalent bond between the HaloTag™ Protein and synthetic ligands.
The covalent bond forms rapidly under physiological conditions, is
highly specific and essentially irreversible, yielding a stable complex
even under denaturing conditions. The HaloTag™ Ligand can carry a
variety of functionalities, including fluorescent labels, affinity
handles and attachments to a solid phase. The flexibility to create
labeled HaloTag™ fusion proteins with a wide range of optical properties
and functions will allow researchers to image and localize labeled
HaloTag™ Protein fusions in live- or fixed-cell populations as well as
isolate and analyze HaloTag™ Protein fusions and protein complexes.
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