|
Download
article
191kb
pdf? |
|
Suppression of Caspase-3 Expression Using the psiSTRIKE™ hMGFP
Vector
Jolanta Vidugiriene, Ph.D., Thomas Yeager, Ph.D.,
Cheryl Bailey, Ph.D., Cindy Sprecher, B.S., Bob Bulleit, Ph.D., and
Doug Storts, Ph.D.
Promega Corporation
The siSTRIKE™ U6 Hairpin Cloning System—hMGFP includes a short hairpin RNA
expression vector for the production of short interfering RNAs. This
vector contains an internal fluorescent marker, the Monster Green™
Fluorescent Protein (hMGFP), to monitor the cellular delivery of these
shRNA constructs. This psiSTRIKE™ hMGFP Vector allows fast and reliable
cloning of hairpin target sequences. The presence of the hMGFP gene
enables transfection efficiency to be determined easily and facilitates
selection of transfected cells by fluorescence-activated cell sorting (FACS®).
Importantly, the expression of hMGFP does not affect gene silencing by
shRNA molecules expressed from the same vector. In this article, we
demonstrate efficient suppression of caspase-3 gene expression using the
psiSTRIKE™ hMGFP Vector.
|
