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In Vitro His-Tag Pull-Down Assay Using MagZ™ Particles
Becky Godat, M.A., and Tonny Johnson, Ph.D.
Promega Corporation
Recently we developed a novel product, the MagZ™ Protein Purification
System, for the purification of expressed his-tagged protein from rabbit
reticulocyte lysate. This protein purification system has a unique
advantage over nickel-based immobilized metal ion affinity
chromatography (IMAC) systems in that it specifically purifies
His-tagged proteins from rabbit reticulocyte lysate without co-purifying
hemoglobin. This article demonstrates that MagZ™ Binding Particles can
be used to develop in vitro His-tag pull-down assays. By using a
His-tagged Id protein attached to MagZ™ Particles, we show that we can
detect the interaction with MyoD.
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