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The MagZ™ System: His-Tagged Protein Purification without
Hemoglobin Contamination
Becky Godat, M.A., Laurie Engel, B.S., and Tonny
Johnson, Ph.D.
Promega Corporation
This novel protein purification system opens up the possibilities for the
expression, purification and functional analysis of His-tagged proteins
expressed in rabbit reticulocyte-based protein expression systems. The
MagZ™ Protein Purification System offers a unique advantage over
nickel-based systems by specifically purifying polyhistidine-tagged
(His-tagged) proteins from rabbit reticulocyte lysate without
co-purification of hemoglobin. Thus, the proteins purified using the
MagZ™ System could be used for several downstream applications, which
are often limited by the presence of hemoglobin in the eluted samples
using other immobilized metal ion affinity chromatography (IMAC)
methods.
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