|
Download
article
191kb
pdf? |
|
Fast, Reliable, High-Quality Midiprep Plasmid Purification
Using the PureYield™ Plasmid Midiprep System
Doug White, M.S., Cheryl Bailey, Ph.D., Hemanth
Shenoi, Ph.D., and
Rex Bitner, Ph.D.
Promega Corporation
This article introduces the PureYield™ Plasmid Midiprep System. The system
is designed to rapidly isolate highly pure plasmid DNA for transfection,
in vitro expression, automated fluorescent DNA sequencing and other
applications. The unique design of the new PureYield™ columns yields
100–200µg of plasmid DNA in less than 30 minutes and elutes in a
concentrated volume, eliminating the need to ethanol precipitate plasmid
DNA prior to use. An Alternative Lysate Clearing Protocol can extend the
system to purify up to 400µg of plasmid DNA. The PureYield™ System can
save up to three-and-a-half hours compared to other plasmid midiprep
methods, freeing researchers to focus on more challenging aspects of
their work.
|
