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Technically Speaking: Cell-Based Caspase Assays: Analyzing the
Data
Martha O'Brien, Ph.D.
Promega Corporation
Caspase assays have been dramatically improved over the past few
years. Previously, cell-based caspase assays were relatively
insensitive and required the preparation of concentrated cell
extracts. Today, caspase assays are extremely sensitive; they can be
performed directly in multiwell cell culture plates, and there are
both luminescent and fluorescent versions of these homogeneous
assays. Along with these improvements, new considerations have
arisen that require careful attention when analyzing data. There are
several features about cell systems that need to be taken into
account when designing an apoptosis paradigm and when analyzing
results from homogeneous caspase assays. Several common questions
regarding the luminescent Caspase-Glo™ Assays highlight these
features.
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