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Efficient Purification of His-Tagged Proteins from Insect and
Mammalian Cells
Natalie Betz, Ph.D.
Promega Corporation
This report demonstrates use of the FastBreak™ Cell Lysis
Reagent and the MagneHis™ Protein Purification System to purify
His-tagged proteins from insect and mammalian cell lysates and
culture media in the presence or absence of serum. The FastBreak™
Reagent, 10X, is added directly to cells in culture, achieving
gentle, efficient lysis in 15 minutes without the need for
centrifugation or mechanical disruption. Lysis can also be achieved
by adding the FastBreak™ Reagent directly to attached or pelleted
cells at a 1X concentration. The MagneHis™ Protein Purification
System can then be used to purify His-tagged proteins present in the
lysate or in culture media. Thus, the combination of the FastBreak™
Reagent and the MagneHis™ Protein Purification System allows
efficient purification of His-tagged proteins from diverse sample
types.
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