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Introducing siLentGene™-2 U6 Hairpin Cloning Systems
Jolanta Vidugiriene, Ph.D., Thomas Yeager, Ph.D.,
Cheryl Bailey, Ph.D., Cindy Sprecher, B.S., and Doug Storts, Ph.D.
Promega Corporation
The siLentGene™-2 U6 Hairpin Cloning Systems (Human) are designed for
convenient and rapid expression of short hairpin siRNA sequences in human
cells. These systems allow PCR-generated products to be directly
transfected into cells for rapid screening of multiple siRNA target
sequences. Once selected, the optimal target sequences can be subcloned
into a predigested psiLentGene™ Basic Vector for transient suppression
of your gene of interest or into vectors with a choice of antibiotic
resistance markers (neomycin, hygromycin or puromycin) for long-term
stable gene suppression.
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