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FastBreak™ Cell Lysis Reagent for Protein Purification
Jude Stevens, B.S., and Gary Kobs, B.S.
Promega Corporation
FastBreak™ Cell Lysis Reagent, 10X is a new cell lysis buffer for use with protein purification systems. The
FastBreak™ Reagent can be added directly to E. coli cultures for gentle, efficient lysis in 10 to 15
minutes without the need for centrifugation or mechanical disruption. We demonstrate the compatibility of the
FastBreak™ Buffer with the MagneHis™ Protein Purification System in both manual and robotic formats.
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