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Purification of In Vitro Transcribed RNA Using the SV Total RNA Isolation System
Jonathan O. Rayner, Ph.D, and Kimberly D. Butler, M.S.
AlphaVax, Inc., 2 Triangle Drive, Research Triangle Park, NC 27709-0307
Purification of RNA following in vitro transcription is required to remove unincorporated nucleotides that can
affect concentration determination, to remove the enzymes usend in the reaction and to exchange buffers. Traditional
methods of RNA purification from in vitro transcription reactions have involved the use of phenol/chloroform extraction
or lithium/chloride precipitation. These methods are often inefficient, and the yield is highly variable. Here we investigate
using the SV Total RNA Isolation System for purification of RNA following in vitro transcription.
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