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Purification of GST-Fusion Proteins by Magnetic Resin-Based MagneGST™ Particles
Marjeta Urh, Ph.D., Don Creswell, B.S., Jacqui Sankbeil, M.S., Dan Simpson, Ph.D., Rod Flemming, Ph.D., Cris Cowan, Ph.D., and Gary Kobs, B.S.
Promega Corporation
The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification
of glutathione-S-transferase (GST) fusion proteins with high efficiency and low background. The magnetic resin-based
batch purification method eliminates the need for multiple centrifugation cycles and provides a simple purification that
can be performed manually or on a robotic platform. Purification of the GST-fusion protein starting from cell pellets
can be accomplished in less than 2 hours from either crude or cleared lysates.
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