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Automating Promega Cell-Based Assays in Multiwell Formats
Tracy Worzella, B.S., and Brad Larson, B.A.
Promega Corporation
In this article, we demonstrate the use of liquid handling workstations
for performing cell-based assays. The feasibility of scale-up and minimal
manipulation required to perform these assays makes them candidates for
implementation on a variety of automated workstations. This article
specifically describes the adaptation of Promega cell-based assays to the
Beckman Coulter Biomek® 2000 and FX workstations in both 96-
and 384-well formats. We have automated the Caspase-Glo™ 3/7 Assay, Apo-ONE®
Homogeneous Caspase-3/7 Assay, CytoTox-ONE™ Homogeneous Membrane
Integrity Assay, CellTiter-Glo® Luminescent Cell Viability
Assay, and CellTiter-Blue™ Cell Viability Assay.
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