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Automation of Genomic DNA Purification Using the MagneSil®
KF, Genomic System
Rex Bitner, Ph.D.1, Susan Koller,
B.S.1, Hemanth Shenoi, Ph.D.1, Kenneth S. Henderson, Ph.D.2, Paulo
Silva, B.S.2, Gregory J. Tsongalis, Ph.D.3
1Promega
Corporation, 2Charles River Laboratories, Wilmington, MA,
3Hartford Hospital, Hartford, CT
DNA analysis techniques have become central in many aspects of modern
basic and clinical research. These methods require high-quality DNA from
diverse sample types that might include blood, tissue, buccal swabs, body
fluids and feces. Traditionally, smaller laboratories have used time- and
labor-intensive manual genomic DNA purification methods, as automated
instruments have been too large, too expensive and not suited for their
lower throughput. This article describes a new system for 1-to 15-sample,
low- to moderate-throughput automated genomic DNA purification. The
MagneSil® KF, Genomic System is designed for use on the Thermo
Electron KingFisher® mL. Using this instrument, DNA
purification takes under 25 minutes. The purified DNA is suitable for PCR,
fluorescent STR, as well as more stringent applications such as multiplex
PCR (e.g., Promega Y Chromosome Deletion Detection System), and SNP
detection systems such as Promega READIT® SNP Genotyping
System.
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