|
Download
article
191kb
pdf? |
|
Introducing Chroma-Luc™ Technology
Brian Almond, Ph.D., Erika Hawkins, M.S., Pete
Stecha, B.S., Denise Garvin, M.S., Aileen Paguio, M.S., Braeden
Butler, B.S., Michael Beck, M.S., Monika Wood, M.S., and Keith Wood,
Ph.D.
Promega Corporation
The Chroma-Luc™ Reporter Vectors encode luciferases that emit
green and red luminescence. Although the luciferases are 98%
identical in amino acid sequence, their peak luminescence
wavelengths are separated by >75nm. Both colors of luminescence
are generated by a single addition of Chroma-Glo™ Reagent, which
has been developed for homogeneous assay of mammalian cells directly
in culture medium. These novel vectors are useful for dual
measurements where closely similar reporter structures are preferred
or where a single reagent addition is desired.
|
