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Automated 96-Well Purification of Genomic DNA from Whole Blood
Susan Koller, B.S., Jacqui Sankbeil, M.S., Hemanth
Shenoi, Ph.D., and
Rex Bitner, Ph.D.
Promega Corporation
Improvements in molecular analysis techniques have changed the
"bottlenecks" in clinical and basic research. Until
recently, analysis required the greatest amount of time and labor.
Today however, the most time-consuming and laborious step is often
DNA purification, especially when high-throughput processing is
required. This article describes two new high-throughput, 96-well,
walkaway automated systems for genomic DNA purification. The
MagneSil® Blood Genomic, Max Yield System is designed
for high-yield genomic DNA purification, purifying 4–9µg of DNA
from 200µl of human whole blood and other samples. The MagneSil®
ONE, Fixed Yield Blood Genomic System is designed to purify a
"fixed DNA yield", 1.0µg ± 50%, from 60µl of whole
blood. Both systems are designed to be implemented on a robotic
liquid handler such as the Beckman Coulter Biomek® FX.
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