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High-Throughput DNA Fragment Purification Using the MagneSil®
Automated 384-Well Clean-Up Systems
Brad Larson, B.A. and Tom Strader, B.S.
Promega Corporation
Automated fluorescent sequencing using energy transfer dyes is the current
method of choice for DNA sequence determination. If not removed prior to
analysis, certain reaction components such as buffer salts and
unincorporated dye-labeled terminators can interfere with data collection
in a capillary-based automated sequencer. Likewise, the presence of
contaminants such as salts, unincorporated dNTPs and primers can interfere
with analysis of PCR fragments by capillary electrophoresis or microarray
spotting. Promega has developed high-throughput, validated, walkaway
systems to remove these contaminants using the Beckman Coulter Biomek®
FX liquid handler and the flexible format of the MagneSil®
Paramagnetic Particles.
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