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siLentGene™ U6 Cassette RNA Interference System: Rapid
Screening of siRNA Targets
By Jolanta Vidugiriene, Ph.D.1, Thomas Yeager,
Ph.D.1, Lin Wang, Ph.D.2, Jiwu Wang, Ph.D.2, Zhaohui Dong, Ph.D.2,
Cynthia Sprecher, B.S.1, and Doug Storts, Ph.D.1
1Promega Corporation, 2Allele Biotechnology and Pharmaceuticals,
Inc.
We have developed an optimized reagent kit for inducing RNA
inferference (RNAi) effects in mammalian cells with siRNA-expressing
DNA cassettes. These cassettes feature an eukaryotic RNA polymerase
III U6 promoter for high efficiency and precision. The expression
cassettes are designed to be synthesized by PCR and transfected into
the desired cell line, making this method suitable for quick
screening of multiple target sites.
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