|
Download
article
232kb
pdf? |
|
Expanding the Capabilities of Plant Genomic DNA Purification
By Susan Koller, B.S.(M.T.), Rex Bitner, Ph.D.,
and Hemanth Shenoi, Ph.D.
Promega Corporation
We describe modifications of two current genomic DNA purification systems
to address researchers' needs for automated plant genomic DNA
purification. We have developed methods in a 96-well format to obtain
greater than 1µg of genomic DNA from leaf or seed as well as methods to
obtain a specific yield of genomic DNA from plant tissue. These methods
improve productivity by providing sufficient DNA for multiple assays from
a single purification or by obtaining a fixed yield of DNA, eliminating
the need to quantitate and normalize before an assay.
Note: On page 3 of this article, we incorrectly stated in Box 1
that 300µl of Lysis Buffer B is used to perform two washes. Instead,
Lysis Buffer A should be used to perform these washes as stated in the Automated
Wizard® Magnetic 96 DNA Plant System: Scaled Up
Protocol for Increased DNA Yield #EP020. We regret the error.
|
