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Introducing GoTaq™ DNA Polymerase: Improved Amplification
With a Choice of Buffers
By Amanda Glebs, B.S., Evelyn Stencel, B.S., and
Kimberly Knoche, Ph.D.
Promega Corporation
GoTaq™ DNA Polymerase, Promega's new formulation of Taq DNA Polymerase,
is supplied with two buffers—Green GoTaq™ Reaction Buffer and
Colorless GoTaq™ Reaction Buffer. DNA amplified using GoTaq™ DNA
Polymerase with Green GoTaq™ Reaction Buffer can be loaded directly onto
agarose gels, eliminating the need to add loading dye or buffer to the PCR
sample before electrophoresis. When PCR samples are not loaded onto a gel
or when the dyes in the Green GoTaq™ Reaction Buffer would interfere
with downstream applications, the Colorless GoTaq™ Reaction Buffer can
be used.
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