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Introducing the Kinase-Glo™ Luminescent
Kinase Assay
By Richard Somberg, Ph.D., Becky Pferdihert, and
Kevin Kupcho, B.S.
Promega Corporation
The Kinase-Glo™ Luminescent Kinase Assay is a homogeneous
method for measuring kinase activity by quantifying a decrease in
ATP levels following kinase activity. The assay can work with
virtually any kinase and substrate combination. The assay is
performed in a single well of a standard multiwell plate by adding
an equal volume of Kinase-Glo™ Reagent and reading luminescence.
The assay produces excellent Z´ values, easily detects known kinase
inhibitors, and provides accurate IC50 values.
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