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Dual-Glo™ Luciferase Assay System: Convenient
Dual Reporter Measurements in 96- and 384-Well Plates
By Erika Hawkins, M.Sc., Braeden Butler, B.S., Michael
Beck, M.S., Michael O'Grady, M.S., Laurie Orr, B.S., and Keith Wood, Ph.D.
Promega Corporation
Promega has developed a new homogeneous reporter assay, the Dual-Glo™
Luciferase Assay System, for monitoring both firefly and Renilla
luciferases expressed in mammalian cells in culture medium in 96- and
384-well plates. The new assay system is similar to the Dual-Luciferase®
Reporter Assay System, except that cell lysis is integrated into the
luminescence chemistry and the luminescence kinetics have been extended
over several hours. The homogeneous assay format allows dual-reporter
assays to be more easily and rapidly performed in multiwell plates by
reducing sample processing requirements and eliminating the need for
reagent injectors in the luminometer.
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