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Apo-ONE™ Homogeneous Caspase-3/7 Assay: Robust,
High-Throughput Apoptosis Detection
By Andrew Niles, M.S.
Promega Corporation
The biological importance of the apoptotic process has gained increased
attention in recent years, ranging from general to specific analysis and
encompassing fields of research as diverse as developmental biology and
toxicology. However, limitations in a number of experimental methods for
apoptosis detection have led to a compromise between apoptotic assay
information content, complexity, throughput and rate of technical success.
The advent of caspase-based proteolysis assays has opened the door to
rapid and definitive confirmation of apoptotic events. Here we describe a
simple, sensitive and flexible assay that is as robust and informative
when automated with state-of-the-art robotics as it is when performed
manually at the benchtop.
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