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CellTiter-Glo™ Luminescent Cell Viability Assay:
Fast, Sensitive and Flexible
By Terry Riss, Ph.D.1, Rich Moravec, B.S.1, Michael Beck,
M.S.1, Rita Hannah, Ph.D.1, Karen Wilson2 and Robert Swanson, Ph.D.3
Promega Corporation1, Trenton College of New Jersey2, Pharmacopeia, Inc.3
Promega's CellTiter-Glo™ Luminescent Cell Viability Assay is a highly
sensitive method for assaying cell proliferation and cytotoxicity. The
assay uses a unique, stable form of luciferase to measure ATP as an
indicator of viable cells. The luminescent signal produced is proportional
to the number of viable cells present in culture. The homogeneous format
of the assay is well suited for high-throughput applications and is
scalable from 96-, 384- and 1536-well formats with little or no loss in
sensitivity. Results can be achieved rapidly, while the extended glow
luminescence feature offers flexibility in processing large batches of
plates.
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