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Demonstration of Immunohistochemical Staining
Using Promega's Anti-ACTIVE® and Apoptosis Antibodies
By Randy Hoffman, B.S.1, Martha O'Brien, Ph.D.1
and Bruce W. Jarvis, Ph.D.2
1Promega Corporation, 2School of Veterinary
Medicine, University of Wisconsin, Madison, WI
This article illustrates tissue staining with Promega polyclonal
antibodies in both mouse and rat systems. We show immunohistochemical data
for Anti-ACTIVE® MAPK, Anti-ACTIVE® JNK,
Anti-ACTIVE® p38 and Anti-ACTIVE® CaM KII pAbs. In
addition, we present work using polyclonal antibodies for the study of
apoptosis (Anti-PARP p85 Fragment pAb, Anti-ACTIVE® Caspase-3
pAb, and Anti-pS473 Akt pAb). Protocols for immunohistochemical
data are provided.
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