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MagneSil™ Paramagnetic Particles: A
High-Throughput PCR Purification System for Microarrays
By Sandra Splinter BonDurant, M.S.1, David
Frisch, Ph.D.1, Matt Lawler, B.S.1, Frederick R.
Blattner, Ph.D.1 and Randy Hoffman, B.S.2
1University of Wisconsin Biotechnology Center, Gene Expression
Center
2Promega Corporation
Differential expression analysis has become a powerful tool for
understanding the genetic basis for pathogenicity of a variety of
microorganisms. High-density microarrays have provided much of the means
for this high-throughput analysis. However, the production and processing
of microarrays often entails the use of nonautomated techniques that
decrease throughput and reproducibility. Here we describe the evaluation
of MagneSil™ Paramagnetic Particles for application in microarray
production, specifically PCR product purification. The highly versatile
silica-coated MagneSil™ Particles can be used to purify nucleic acids
from a variety of starting materials. Their properties also make them
highly amenable to automation, a critical characteristic for microarray
production. Initial PCR purification studies were done in 96 well format
using Beckman Coulter's Biomek® FX instrument and the Wizard®
MagneSil™ PCR Clean-Up System. Purified PCR products amplified from E.
coli genes were evaluated for purity, recovery, microarray spot
morphology and hybridization efficiency.
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