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Optimized Gene Expression with the T7 Sample
System
By Cassandra Brouette, B.S., Natalie Betz, Ph.D. and Gary
Kobs, B.S.
Promega Corporation
The T7 Sample System contains four unique in vitro translation systems for
one-step gene expression from circular and linear plasmid DNA templates,
as well as nonpurified PCR products. The system contains samples of TNT®
T7 Quick for PCR DNA, TNT® T7 Quick Coupled Transcription/Translation
System, TNT® T7 Coupled Wheat Germ Extract System and the E. coli T7 S30
Extract System for Circular DNA. This article discusses the influence of
vector and insert sequences on optimal gene expression in both eukaryotic
and prokaryotic in vitro translation systems as shown using these four
gene expression systems.
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