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High-Quality In Vitro Expression Cloning Using the
Gold TNT® T7 Express System
By Olaf Stemmann, Ph.D., Hui Zou, Ph.D. and Marc
Kirschner, Ph.D.
Harvard Medical School
The Gold TNT® T7 Express 96 System is a recent enhancement to
Promega's line of in vitro expression products. The system is composed of
components similar to the original TNT® Coupled
Transcription/Translation Systems, with the Gold TNT® System
components meeting a higher specification for luciferase activity. In
addition, the Gold System components allow for large-scale screening
assays through the convenience of a 96 well plate configuration. The
authors used the Gold TNT® T7 Express 96 System for in vitro
expression cloning (IVEC) to identify a gene that produces a substrate of
separase.
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