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High-Throughput Purification Using the SV 96
Total RNA Isolation System
By Terri Grunst, B.S.
Promega Corporation
This article introduces the SV 96 RNA Isolation System. The system is used to purify
high-quality total RNA with a flexible method that can be performed manually or automated
on a liquid handling workstation such as the Beckman Biomek® 2000 or the
Biomek® FX. The unique design of the SV 96 Total RNA Isolation System
eliminates waste handling, includes a DNase treatment step to eliminate genomic DNA and
allows consistent and reliable recovery of total RNA from tissue culture cells. The
purified RNA is functionally tested in RT-PCR and results are shown to be consistent with
those obtained using other RNA purification methods.
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