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New Synthetic Renilla Gene and Assay
System Increase Expression, Reliability and Sensitivity
By Yao Zhuang, Ph.D., Braeden Butler, B.S., Erika Hawkins, M.S., Aileen
Paguio, M.S., Laurie Orr, B.S., Monika Wood, M.S., and Keith V. Wood, Ph.D.
Promega Corporation
A new synthetic gene encoding Renilla luciferase has been designed to provide
greater expression efficiency and reliability as a genetic reporter. The synthetic Renilla
gene (hRluc) is codon optimized for mammalian cells and is designed to minimize
unintended mechanisms of genetic regulation. The gene is provided in seven different
vector configurations to support applications both as a control reporter and a primary
reporter. These vectors can be used either with the Dual-Luciferase® Reporter
Assay System or the newly developed Renilla Luciferase Reporter Assay System. The
new Renilla Luciferase Reporter Assay System provides high sensitivity due to a
novel formulation that virtually eliminates background luminescence common to conventional
assays. This new reagent provides rapid and linear quantitation over eight logs of enzyme
concentration.
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