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Technically Speaking: Promega RT-PCR Systems
Explained
By Hanan Abramovici, M.S.
Promega Corporation
PCR has proven to be one of the most flexible and powerful
techniques in modern molecular biology. Reverse transcription-PCR (RT-PCR) adds a new
dimension, coupling the conversion of RNA into complementary DNA (cDNA)by viral reverse
transcriptases with the amplification of the cDNA by thermostable polymerases. (More
information on this topic is available online at: www.promega.com/amplification/.) Promega
offers two systems for RT-PCR: The Access RT-PCR System and the Reverse Transcription
System.
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