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FluoroTectTM GreenLys in
vitro Translation Labeling System
Gary Kobs, B.S., Robin Hurst, M.S., Natalie Betz, Ph.D., and Becky
Godat,
B.S.
Promega Corporation
The FluoroTectTM GreenLys in vitro Translation Labeling System is
the first commercially available fluorescent technology for labeling in vitro translation
products. The FluoroTectTM System uses a modified charged lysine transfer RNA
(tRNA) labeled with the fluorophore, BODIPY®-FL. The modified lysine tRNA is
used efficiently in both prokaryotic and eukaryotic in vitro translation systems. Data is
obtained in a few minutes by the detection of the fluorescently labeled proteins
"in-gel" with laser-based fluorescent gel scanning. This feature circumvents the
multiple manipulations associated with traditional isotopic and nonisotopic incorporation
and detection methodologies.
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