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RNasin® Ribonuclease Inhibitor Part
I: Characterization of the Protein
John Shultz, Ph.D., Robin Hurst, M.S., and Natalie Betz, Ph.D.
Promega Corporation
Ribonucleases (RNases) are ubiquitous enzymes that can detrimentally affect RNA
isolation in the laboratory. Many applications such as RT-PCR and in vitro transcription
and translation may be affected as well. RNase can renature after autoclaving and is
difficult to inactivate. Many RNase inhibitors are available commercially. Promega's
RNasin® Ribonuclease Inhibitor, one of the first introduced, is exceptionally
effective at controlling RNase activity during molecular biology procedures.
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