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The Poly(A) mRNA Detection System
Donna Leippe, Ph.D., Ken Lewis, Ph. D., John
Shannonhouse, M.S., John
Shultz, Ph.D., and Scott Weatherbee*, Ph.D.
Promega Corporation and *Memorial Sloan-Kettering Cancer Center, New York, NY.
The Poly(A) mRNA Detection System is a luciferase-based system designed for the
specific and sensitive detection of polyadenylated messenger RNA. Detection is based on a
series of enzymatic reactions resulting in a light signal proportional to the amount of
poly(A) mRNA in the sample. The system is specific; minimal signal is generated from
ribosomal or transfer RNAs. The system is also sensitive and can detect picogram levels of
mRNA, levels that cannot be detected by conventional methods such as
spectrophotometry.
This also minimizes the amount of valuable sample committed to measurement purposes. This
article demonstrates the linearity, specificity and sensitivity of this new system.
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