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Tools to Study the Activation of CaM KII in
Neuronal Functions
Said Goueli, Ph.D.
Promega Corporation
Protein kinases play an important role in cell signaling and in various cellular
functions such as neurotransmitter release, long-term potentiation, the mediation of
hormonal actions and in regulation of ion channel responses to extracellular signals. The
availability of the appropriate tools to study protein kinases in neuronal signaling will
certainly hasten the pace of discoveries and understanding of the mechanisms involved in
neuronal growth, signaling and neurodegeneration. We have developed two such reagents, the
SignaTECT® CaM KII System and Anti-ACTIVE® CaM KII pAb, Rabbit,
(pT286). The SignaTECT® System can be used to detect the kinase
activity and the activation of CaM KII with high sensitivity (i.e., nanogram level of
enzyme). Anti-ACTIVE® CaM KII pAb, Rabbit, (pT286) can detect the
phosphorylation of a threonine residue that is critical to CaM KII regulation as a result
of enzyme autophosphorylation under increased calcium signaling. These reagents will allow
researchers to study CaM KII signaling in various model systems including neuronal
cultures, PC12 cells and brain tissue.
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