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Technically Speaking: M-MLV Reverse
Transcriptase, RNase H Minus
Doug Barron, M.S.
Promega Corporation
During cDNA sythesis, the RNase H activity of reverse transcriptases can interfere with
the enzymes' ability to generate full-length cDNA from long RNA templates. Use of an RNase
H minus reverse transcriptase (such as engineered forms M-MLV RT) increases the percentage
of full-length cDNA synthesized and is recommended when generating full-length cDNA
libraries.
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