|
Download
article
536kb
pdf? |
|
CaspACE™ FITC-VAD-FMK In Situ Marker
for Apoptosis: Applications for Flow Cytometry
1Matt Sylte, DVM, M.S., 2Martha O'Brien, Ph.D., 3Thomas
J. Inzana, Ph.D. and 1Chuck Czuprynski, Ph.D.
1Department of Pathobiological Sciences, School of Veterinary Medicine,
University of Wisconsin, Madison, WI, 2Promega Corporation, 3Department
of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary
Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA.
Cells frequently respond to environmental changes or insults by undergoing apoptosis.
Apoptosis is an essential component of the organism's repertoire of cellular responses,
whether it is triggered by a genetic program for development or induced by an external
factor. Researchers performing cell-based assays in a high-throughput screening (HTS)
capacity are frequently interested in the apoptotic response. In cell-based assays for
cancer drugs, apoptosis may be the desired response; conversely, in screens designed for
therapeutic reagents for various degenerative diseases, the ability to inhibit apoptosis
may be desired. Most assays for apoptosis are not readily adapted to HTS, however. Promega
has recently developed a useful marker for apoptosis that can be added directly to cells
in culture. The simple add, wash and analyze format makes this apoptosis marker easily
adaptable for use in high-throughput screening systems.
|
